Cloning and characterization of a lysosomal phospholipase A2, 1-O-acylceramide synthase
M Hiraoka, A Abe, JA Shayman - Journal of biological chemistry, 2002 - ASBMB
Recently, a novel enzyme, 1-O-acylceramide synthase (ACS), was purified and
characterized from bovine brain. This enzyme has both calcium-independent phospholipase
A 2 and transacylase activities. The discovery of this enzyme led us to propose a new
pathway for ceramide metabolism in which the sn-2-acyl group of either
phosphatidylethanolamine or phosphatidylcholine is transferred to the 1-hydroxyl group of
ceramide. In this study, the partial amino acid sequences from the purified enzyme revealed …
characterized from bovine brain. This enzyme has both calcium-independent phospholipase
A 2 and transacylase activities. The discovery of this enzyme led us to propose a new
pathway for ceramide metabolism in which the sn-2-acyl group of either
phosphatidylethanolamine or phosphatidylcholine is transferred to the 1-hydroxyl group of
ceramide. In this study, the partial amino acid sequences from the purified enzyme revealed …
